E. coli <FONT FACE=Symbol>a</FONT>-hemolysin: a membrane-active protein toxin
نویسندگان
چکیده
منابع مشابه
E . coli α - hemolysin : a membrane - active protein toxin
α-Hemolysin is synthesized as a 1024-amino acid polypeptide, then intracellularly activated by specific fatty acylation. A second activation step takes place in the extracellular medium through binding of Ca2+ ions. Even in the absence of fatty acids and Ca2+ HlyA is an amphipathic protein, with a tendency to self-aggregation. However, Ca2+-binding appears to expose hydrophobic patches on the p...
متن کاملAcylation of Escherichia coli hemolysin: a unique protein lipidation mechanism underlying toxin function.
The pore-forming hemolysin (HlyA) of Escherichia coli represents a unique class of bacterial toxins that require a posttranslational modification for activity. The inactive protoxin pro-HlyA is activated intracellularly by amide linkage of fatty acids to two internal lysine residues 126 amino acids apart, directed by the cosynthesized HlyC protein with acyl carrier protein as the fatty acid don...
متن کاملProduction of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System
The ultimate aim of biochemistry is to decipher how events occur inside of living cells or organisms. The living cell must also be regarded as the ideal model system for any biochemical application, as most physiological targets are found inside living cells. The cell envelope of the Gram-negative bacterium Escherichia coli is a complex structure and many of the proteins found in the bacterial ...
متن کاملCloning and expression of Brucella outer membrane protein 36kDa (OMP2b) in E. coli
Background & Objective: Brucellosis is an important zoonotic disease of economic significance. Brucella species are gram-negative, facultative intracellular bacteria, and are capable of replicating in the phagosomes of macrophages. They cause infection in several animal species and humans. Prevention of new diseases and diagnosis of cases infected with the organism are both essential for eradic...
متن کاملE. coli Hemolysin E (HlyE, ClyA, SheA) X-Ray Crystal Structure of the Toxin and Observation of Membrane Pores by Electron Microscopy
Hemolysin E (HlyE) is a novel pore-forming toxin of Escherichia coli, Salmonella typhi, and Shigella flexneri. Here we report the X-ray crystal structure of the water-soluble form of E. coli HlyE at 2.0 A resolution and the visualization of the lipid-associated form of the toxin in projection at low resolution by electron microscopy. The crystal structure reveals HlyE to be the first member of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 1998
ISSN: 0100-879X
DOI: 10.1590/s0100-879x1998000800002